Instrumental Method of Analysis : Unit 1 Notes : Bpharm

Download high-quality and well-structured Bpharm 1st-Semester notes in pdf format. The notes are in easy language and easy to remember covering all the topics of the syllabus.

Unit 1

UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.

Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications – Spectrophotometric titrations, Single component and multi component analysis

Fluorimetry :

Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications.

Instrumental Method of Analysis : Unit 1 Notes : Bpharm

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